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KD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1730
Application
WB, FC, ICC
Uniprot Id
P26368
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-U2AF2 antibody (Cat#ABG1730). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-U2AF2 antibody (Cat#ABG1730, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-U2AF2 antibody (Cat#ABG1730). U2AF2 expression in wild-type (WT) and U2AF2 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-U2AF2 antibody (Cat#ABG1730, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of U2AF2 expression in HepG2 cells using anti-U2AF2 antibody (Cat#ABG1730, 1:2,000). Green, isotype control; red, U2AF2.
  • Immunocytochemical staining of HepG2 cells with anti-U2AF2 antibody (Cat#ABG1730, 1:1,000). Nuclei were stained blue with DAPI; U2AF2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1730-100 3 days 100 µL $499.00
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ABG1730-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-U2AF2 Rabbit Monoclonal Antibody
Accession (Primary)P26368
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB2465
Immunogen
A synthesized peptide derived from human U2AF65
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 54 kDa , observed , 54 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( P26368 )

Gene NameU2AF2
Other Gene Names
U2AF2; U2 Small Nuclear RNA Auxiliary Factor 2; U2AF65; U2 SnRNP Auxiliary Factor Large Subunit; U2 Small Nuclear Ribonucleoprotein Auxiliary Factor (65kD); U2 (RNU2) Small Nuclear RNA Auxiliary Factor 2; Splicing Factor U2AF 65 KDa Subunit; U2 Auxiliary Factor 65 KDa Subunit; HU2AF65; Splicing Factor U2AF 65 KD Subunit; HU2AF(65); DEVDFB
About Gene Symbol
U2AF2 (U2 Small Nuclear RNA Auxiliary Factor 2) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied t
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