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KD-Validated Anti-SMC4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1744
Application
WB, FC, ICC
Uniprot Id
Q9NTJ3
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-SMC4 antibody (Cat#ABG1744). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMC4 antibody (Cat#ABG1744, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-SMC4 antibody (Cat#ABG1744). SMC4 expression in wild type (WT) and SMC4 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMC4 antibody (Cat#ABG1744, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of SMC4 expression in HT-1080 cells using anti-SMC4 antibody (Cat#ABG1744, 1:2,000). Green, isotype control; red, SMC4.
  • Immunocytochemical staining of HT-1080 cells with anti-SMC4 antibody (Cat#ABG1744, 1:1,000). Nuclei were stained blue with DAPI; SMC4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1744-100 3 days 100 µL $499.00
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ABG1744-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-SMC4 Rabbit Monoclonal Antibody
Accession (Primary)Q9NTJ3
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB2400
Immunogen
A synthesized peptide derived from human SMC4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 147 kDa , observed , 180 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q9NTJ3 )

Gene NameSMC4
Other Gene Names
SMC4; Structural Maintenance Of Chromosomes 4; HCAP-C; SMC4L1; CAP-C; Structural Maintenance Of Chromosomes Protein 4; Chromosome-Associated Polypeptide C; SMC Protein 4; SMC-4; CAPC; SMC4 (Structural Maintenance Of Chromosomes 4, Yeast)-Like 1; SMC4 Structural Maintenance Of Chromosomes 4-Like 1 (Yeast); SMC4 Structural Maintenance Of Chromosomes 4-Like 1; XCAP-C Homolog
About Gene Symbol
SMC4 (Structural Maintenance Of Chromosomes 4) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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