KD-Validated Anti-Ubiquitin Specific Peptidase 5 Rabbit Monoclonal Antibody

Western blotting analysis using anti-Ubiquitin specific peptidase 5 antibody (Cat#ABG1747). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ubiquitin specific peptidase 5 antibody (Cat#ABG1747, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Ubiquitin specific peptidase 5 antibody (Cat#ABG1747). Ubiquitin specific peptidase 5 expression in wild type (WT) and Ubiquitin specific peptidase 5 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Ubiquitin specific peptidase 5 antibody (Cat#ABG1747, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Ubiquitin specific peptidase 5 expression in HepG2 cells using anti-Ubiquitin specific peptidase 5 antibody (Cat#ABG1747, 1:2,000). Green, isotype control; red, Ubiquitin specific peptidase 5.

Immunocytochemical staining of HepG2 cells with anti-Ubiquitin specific peptidase 5 antibody (Cat#ABG1747, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.