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KD-Validated Anti-Trophoblast Glycoprotein Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1751
Application
WB, FC, ICC
Uniprot Id
Q13641
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Trophoblast glycoprotein antibody (Cat#ABG1751). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Trophoblast glycoprotein antibody (Cat#ABG1751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-trophoblast glycoprotein antibody (Cat#ABG1751). Trophoblast glycoprotein expression in wild-type (WT) and trophoblast glycoprotein (TPBG) shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-trophoblast glycoprotein antibody (Cat#ABG1751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Trophoblast glycoprotein expression in HAP-1 cells using anti-Trophoblast glycoprotein antibody (Cat #ABG1751, 1:2,000). Green, isotype control; red, Trophoblast glycoprotein.
  • Immunocytochemical staining of HAP-1 with anti-trophoblast glycoprotein antibody (Cat#ABG1751, 1:1,000). Nuclei were stained blue with DAPI; Trophoblast glycoprotein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1751-100 3 days 100 µL $499.00
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ABG1751-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Trophoblast Glycoprotein Rabbit Monoclonal Antibody
Accession (Primary)Q13641
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1990
Immunogen
A synthesized peptide derived from human 5T4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 46 kDa , o bserved , 72 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q13641 )

Gene NameTPBG
Other Gene Names
TPBG; Trophoblast Glycoprotein; 5T4; 5T4 Oncofetal Trophoblast Glycoprotein; Wnt-Activated Inhibitory Factor 1; 5T4 Oncotrophoblast Glycoprotein; 5T4 Oncofetal Antigen; 5T4-AG; WAIF1; M6P1; 5T4AG
About Gene Symbol
TPBG (Trophoblast Glycoprotein) is a protein kinase and signaling regulator involved in Wnt/β-catenin signaling and adaptive immunity. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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