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KD-Validated Anti-UBE2L3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1759
Application
WB, FC, ICC
Uniprot Id
P68036
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-UBE2L3 antibody (Cat#ABG1759). Total cell lysates (5 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2L3 antibody (Cat#ABG1759, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-UBE2L3 antibody (Cat#ABG1759). UBE2L3 expression in wild-type (WT) and UBE2L3 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-UBE2L3 antibody (Cat#ABG1759, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of UBE2L3 expression in HepG2 cells using anti-UBE2L3 antibody (Cat#ABG1759, 1:2,000). Green, isotype control; red, UBE2L3.
  • Immunocytochemical staining of HepG2 cells with anti-UBE2L3 antibody (Cat#ABG1759, 1:1,000). Nuclei were stained blue with DAPI; UBE2L3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1759-100 3 days 100 µL $499.00
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ABG1759-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-UBE2L3 Rabbit Monoclonal Antibody
Accession (Primary)P68036
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB1875
Immunogen
A synthesized peptide derived from human UBE2L3
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 18 kDa , observed , 17 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P68036 )

Gene NameUBE2L3
Other Gene Names
UBE2L3; Ubiquitin Conjugating Enzyme E2 L3; UBCH7; Ubiquitin-Conjugating Enzyme E2 L3; E2 Ubiquitin-Conjugating Enzyme L3; Ubiquitin-Conjugating Enzyme E2-F1; Ubiquitin Carrier Protein L3; Ubiquitin-Protein Ligase L3; L-UBC; Ubiquitin-Conjugating Enzyme E2L 3; Ubiquitin Conjugating Enzyme E2L 3; Ubiquitin-Conjugating Enzyme UBCH7; EC 2.3.2.23; EC 6.3.2.19; E2-F1; UbcM4; UBCE7; UbcH7
About Gene Symbol
UBE2L3 (Ubiquitin Conjugating Enzyme E2 L3) is a ubiquitin E3 ligase involved in ubiquitin-proteasome pathway. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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