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KD-Validated Anti-THO Complex Subunit 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1763
Application
WB, FC, ICC
Uniprot Id
Q96FV9
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-THO complex subunit 1 antibody (Cat#ABG1763). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-THO complex subunit 1 antibody (Cat#ABG1763, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-THO complex subunit 1 antibody (Cat#ABG1763). THO complex subunit 1 expression in wild-type (WT) and THO complex subunit 1 (THOC1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-THO complex subunit 1 antibody (Cat#ABG1763, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of THO complex subunit 1 expression in HepG2 cells using anti-THO complex subunit 1 antibody (Cat#ABG1763, 1:2,000). Green, isotype control; red, THO complex subunit 1.
  • Immunocytochemical staining of HepG2 cells with anti-THO complex subunit 1 antibody (Cat#ABG1763, 1:1,000). Nuclei were stained blue with DAPI; THO complex subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1763-100 3 days 100 µL $499.00
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ABG1763-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-THO Complex Subunit 1 Rabbit Monoclonal Antibody
Accession (Primary)Q96FV9
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB2205
Immunogen
A synthesized peptide derived from human Nuclear Matrix Protein p84
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 76 kDa , observed , 76 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( Q96FV9 )

Gene NameTHOC1
Other Gene Names
THOC1; THO Complex Subunit 1; HPR1; P84; Nuclear Matrix Protein P84; THO Complex 1; HTREX84; P84N5; Tho1; DFNA86
About Gene Symbol
THOC1 (THO Complex Subunit 1) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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