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KD-Validated Anti-Timeless Circadian Regulator Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1767
Application
WB, FC, ICC
Uniprot Id
Q9UNS1
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Timeless circadian regulator antibody (Cat#ABG1767). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Timeless circadian regulator antibody (Cat#ABG1767, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-timeless circadian regulator antibody (Cat#ABG1767). Timeless circadian regulator expression in wild-type (WT) and timeless circadian regulator (TIMELESS) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-timeless circadian regulator antibody (Cat#ABG1767, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Timeless circadian regulator expression in HepG2 cells using anti-Timeless circadian regulator antibody (Cat#ABG1767, 1:2,000). Green, isotype control; red, Timeless circadian regulator.
  • Immunocytochemical staining of HepG2 cells with anti-Timeless circadian regulator antibody (Cat#ABG1767, 1:1,000). Nuclei were stained blue with DAPI; Timeless circadian regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1767-100 3 days 100 µL $499.00
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ABG1767-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Timeless Circadian Regulator Rabbit Monoclonal Antibody
Accession (Primary)Q9UNS1
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB2665
Immunogen
A synthesized peptide derived from human Timeless
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 139 kDa , observed , 150 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB):1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q9UNS1 )

Gene NameTIMELESS
Other Gene Names
TIMELESS; Timeless Circadian Regulator; HTIM; TIM1; TIM; Timeless Circadian Clock 1; Protein Timeless Homolog; Timeless (Drosophila) Homolog; Timeless Homolog (Drosophila); Tof1 Homolog (S. Cerevisiae); Timeless Homolog; Tof1 Homolog; TIMELESS1; FASPS4
About Gene Symbol
TIMELESS (Timeless Circadian Regulator) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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