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KD-Validated Anti-Prolyl 4-Hydroxylase Subunit Beta Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1780
Application
WB, FC, ICC
Uniprot Id
P07237
Reactivity
Human
 
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  • Western blotting analysis using anti-P4HB antibody (Cat#ABG1780). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-P4HB antibody (Cat#ABG1780, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-P4HB antibody (Cat#ABG1780). P4HB expression in wild type (WT) and P4HB shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-P4HB antibody (Cat#ABG1780, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of P4HB expression in HepG2 cells using anti-P4HB antibody (Cat #ABG1780, 1:2,000). Green, isotype control; red, P4HB.
  • Immunocytochemical staining of HepG2 cells with anti-P4HB antibody (Cat#ABG1780, 1:1000). Nuclei were stained blue with DAPI; P4HB was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1780-100 3 days 100 µL $499.00
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ABG1780-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Prolyl 4-Hydroxylase Subunit Beta Mouse Monoclonal Antibody
Accession (Primary)P07237
Host SpeciesMouse
ClonalityMonoclonal
Clone Names24GB2780
Immunogen
Recombinant protein of human PDI
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 57 kDa , o bserved , 57 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P07237 )

Gene NameP4HB
Other Gene Names
P4HB; Prolyl 4-Hydroxylase Subunit Beta; PDIA1; PDI; P4Hbeta; ERBA2L; PROHB; PO4HB; PO4DB; DSI; GIT; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide; Protein Disulfide Isomerase Family A, Member 1; Protein Disulfide Isomerase-Associated 1; Cellular Thyroid Hormone-Binding Protein; Prolyl 4-Hydroxylase, Beta Polypeptide; Collagen Prolyl 4-Hydroxylase Beta; Protein Disulfide-Isomerase; EC 5.3.4.1; P55; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase; Thyroid Hormone Binding Protein P55) ; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase (Proline 4-Hydroxylase), Beta Polypeptide (Protein Disulfide Isomerase-Associated 1); Protein Disulfide Isomerase/Oxidoreductase; Glutathione-Insulin Transhydrogenase; Thyroid Hormone-Binding Protein P55; Testicular Secretory Protein Li 32; Protocollagen Hydroxylase; CLCRP1; PHDB
About Gene Symbol
P4HB (Prolyl 4-Hydroxylase Subunit Beta) is a collagen extracellular matrix protein involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular
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