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KD-Validated Anti-Cyclin Dependent Kinase 4 Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1782
Application
WB, ICC
Uniprot Id
P11802
Reactivity
Human, Mouse, Rat
Isotype
Mouse IgG2a kappa
 
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  • Western blotting analysis using anti-Cyclin dependent kinase 4 antibody (Cat#ABG1782). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cyclin dependent kinase 4 antibody (Cat#ABG1782, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-Cyclin dependent kinase 4 antibody (Cat#ABG1782). Cyclin dependent kinase 4 expression in wild type (WT) and Cyclin dependent kinase 4 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Cyclin dependent kinase 4 antibody (Cat#ABG1782, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-cyclin dependent kinase 4 antibody (Cat#ABG1782, 1:1,000). Nuclei were stained blue with DAPI; Cyclin dependent kinase 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1782-100 3 days 100 µL $499.00
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ABG1782-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin Dependent Kinase 4 Mouse Monoclonal Antibody
Accession (Primary)P11802
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG2a kappa
Clone Names24GB2800
Immunogen
Recombinant protein of human CDK4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 34 kDa , observed , 33 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:2,500-1:5,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P11802 )

Gene NameCDK4
Other Gene Names
CDK4; Cyclin Dependent Kinase 4; PSK-J3; Cell Division Protein Kinase 4; Cyclin-Dependent Kinase 4; EC 2.7.11.22; EC 2.7.11; CMM3
About Gene Symbol
CDK4 (Cyclin Dependent Kinase 4) is a protein kinase and signaling regulator involved in cell cycle regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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