KD-Validated Anti-MITF Mouse Monoclonal Antibody

Western blotting analysis using anti-Melanocyte inducing transcription factor antibody (Cat#ABG1787). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Melanocyte inducing transcription factor antibody (Cat#ABG1787, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Western blotting analysis using anti-melanocyte inducing transcription factor antibody (Cat#ABG1787). Melanocyte inducing transcription factor expression in wild-type (WT) and melanocyte inducing transcription factor (MITF) shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-melanocyte inducing transcription factor antibody (Cat#ABG1787, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Immunocytochemical staining of HepG2 cells with anti-Melanocyte inducing transcription factor antibody(Cat#ABG1787, 1:1,000). Nuclei were stained blue with DAPI; Melanocyte inducing transcription factor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.