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KD-Validated Anti-Phosphoglycerate Kinase 1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1789
Application
WB, ICC
Uniprot Id
P00558
Reactivity
Human, Mouse, Rat
Isotype
Mouse IgG
 
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  • Western blotting analysis using anti-Phosphoglycerate kinase 1 antibody (Cat#ABG1789). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phosphoglycerate kinase 1 antibody (Cat#ABG1789, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-phosphoglycerate kinase 1 antibody (Cat#ABG1789). Phosphoglycerate kinase 1 expression in wild-type (WT) and phosphoglycerate kinase 1 (PGK1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phosphoglycerate kinase 1 antibody (Cat#ABG1789, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-Phosphoglycerate kinase 1 antibody(Cat#ABG1789, 1:1,000). Nuclei were stained blue with DAPI; Phosphoglycerate kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1789-100 3 days 100 µL $499.00
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ABG1789-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Phosphoglycerate Kinase 1 Mouse Monoclonal Antibody
Accession (Primary)P00558
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG
Clone Names24GB2935
Immunogen
Recombinant protein of human PGK1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 45 kDa, observed, 44 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P00558 )

Gene NamePGK1
Other Gene Names
PGK1; Phosphoglycerate Kinase 1; Cell Migration-Inducing Gene 10 Protein; Primer Recognition Protein; EC 2.7.2.3; PRP; PGKA; Epididymis Secretory Sperm Binding Protein Li 68p; HEL-S-68p; MIG10
About Gene Symbol
PGK1 (Phosphoglycerate Kinase 1) is a protein kinase and signaling regulator involved in cell migration and invasion. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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