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KD-Validated Anti-Phospho-eIF2 alpha (Ser51) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1795
Application
WB, FC, ICC
Uniprot Id
P05198
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#ABG1795). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-eIF2 alpha (Ser51) antibody (Cat#ABG1795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-phospho-eIF2 alpha (Ser51) antibody (Cat#ABG1795). Phospho-eIF2 alpha (Ser51) expression in wild-type (WT) and EIF2S1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-eIF2 alpha (Ser51) antibody (Cat#ABG1795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of mTOR expression in HepG2 cells using anti-mTOR antibody (Cat #63038, 1:2,000). Green, isotype control; red, mTOR.
  • Immunocytochemical staining of HT-1080 cells with anti-Phospho-eIF2 alpha (Ser51) antibody (Cat#ABG1795, 1:1,000). Nuclei were stained blue with DAPI; Phospho-eIF2 alpha (Ser51) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1795-100 3 days 100 µL $499.00
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ABG1795-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Phospho-eIF2 alpha (Ser51) Rabbit Monoclonal Antibody
Accession (Primary)P05198
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB3905
Immunogen
A synthesized peptide derived from human Phospho-eIF2 alpha (Ser51)
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 36 kDa, observed, 36 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P05198 )

Gene NameEIF2S1
Other Gene Names
EIF2S1; Eukaryotic Translation Initiation Factor 2 Subunit Alpha; EIF-2alpha; EIF2A; Eukaryotic Translation Initiation Factor 2, Subunit 1 Alpha, 35kDa; Eukaryotic Translation Initiation Factor 2 Subunit 1; EIF-2-Alpha; EIF2-Alpha; EIF-2A; EIF2; Eukaryotic Translation Initiation Factor 2, Subunit 1 (Alpha, 35kD ); EIF-2
About Gene Symbol
EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit Alpha) is a protein kinase and signaling regulator involved in translational regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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