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KD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1798
Application
WB, FC, ICC
Uniprot Id
P26358
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#ABG1798). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#ABG1798, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-DNA methyltransferase 1 antibody (Cat#ABG1798). DNA methyltransferase 1 expression in wild type (WT) and DNA methyltransferase 1 (DNMT1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA methyltransferase 1 antibody (Cat#ABG1798, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of DNA methyltransferase 1 expression in HepG2 cells using anti-DNA methyltransferase 1 antibody (Cat#ABG1798, 1:2,000). Green, isotype control; red, DNA methyltransferase 1.
  • Immunocytochemical staining of HepG2 cells with DNA methyltransferase 1 antibody (Cat#ABG1798, 1:1,000). Nuclei were stained blue with DAPI; DNA methyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1798-100 3 days 100 µL $499.00
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ABG1798-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-DNA Methyltransferase 1 Rabbit Monoclonal Antibody
Accession (Primary)P26358
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB5135
Immunogen
A synthesized peptide derived from human Dnmt1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 183 kDa, o bserved , 200 k Da
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
WesternBlotting (WB):1:1,000-1:5,000 FlowCytometry(FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P26358 )

Gene NameDNMT1
Other Gene Names
DNMT1; DNA Methyltransferase 1; CXXC9; MCMT; DNMT; DNA (Cytosine-5-)-Methyltransferase 1; DNA (Cytosine-5)-Methyltransferase 1; CXXC-Type Zinc Finger Protein 9; DNA Methyltransferase HsaI; DNA MTase HsaI; EC 2.1.1.37; M.HsaI; AIM; ADCADN; HSN1E; Dnmt1
About Gene Symbol
DNMT1 (DNA Methyltransferase 1) is a methyltransferase involved in gene transcription and regulation. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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