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KD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1804
Application
WB, FC, ICC
Uniprot Id
P14635
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-cyclin B1 antibody (Cat#ABG1804). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin B1 antibody (Cat#ABG1804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-cyclin B1 antibody (Cat#ABG1804). Cyclin B1 expression in wild-type (WT) and cyclin B1 (CCNB1) shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin B1 antibody (Cat#ABG1804, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Cyclin B1 expression in HeLa cells using anti-Cyclin B1 antibody (Cat#ABG1804, 1:2,000). Green, isotype control; red, Cyclin B1.
  • Immunocytochemical staining of Hela cells with Cyclin B1 antibody (Cat#ABG1804, 1:1,000). Nuclei were stained blue with DAPI;Cyclin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium.Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1804-100 3 days 100 µL $499.00
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ABG1804-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin B1 Rabbit Monoclonal Antibody
Accession (Primary)P14635
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB4300
Immunogen
A synthesized peptide derived from human Cyclin B1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 48 kDa, observed, 55 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P14635 )

Gene NameCCNB1
Other Gene Names
CCNB1; Cyclin B1; CCNB; G2/Mitotic-Specific Cyclin-B1; G2/Mitotic-Specific Cyclin B1
About Gene Symbol
CCNB1 (Cyclin B1) is a protein kinase and signaling regulator involved in cell cycle regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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