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KD-Validated Anti-G3BP1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1834
Application
WB, FC, ICC
Uniprot Id
Q13283
Reactivity
Human, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-G3BP1 antibody (Cat#ABG1834). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-G3BP1 antibody (Cat#ABG1834, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-G3BP1 antibody (Cat#ABG1834). G3BP1 expression in wild type (WT) and G3BP1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-G3BP1 antibody (Cat#ABG1834, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of G3BP1 expression in HepG2 cells using anti-G3BP1 antibody (Cat#ABG1834, 1:2,000). Green, isotype control; red, G3BP1.
  • Immunocytochemical staining of HepG2 cells with anti-G3BP1 antibody (Cat#ABG1834, 1:1,000). Nuclei were stained blue with DAPI; G3BP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1834-100 3 days 100 µL $499.00
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ABG1834-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-G3BP1 Rabbit Monoclonal Antibody
Accession (Primary)Q13283
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB4575
Immunogen
A synthesized peptide derived from human G3BP
Species Reactivity
Reacts WithHuman, Rat
Calculated MW
Predicted, 52 kDa,observed, 68 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q13283 )

Gene NameG3BP1
Other Gene Names
G3BP1; G3BP Stress Granule Assembly Factor 1; G3BP; HDH-VIII; Ras-GTPase-Activating Protein SH3-Domain-Binding Protein; GTPase Activating Protein (SH3 Domain) Binding Protein 1; Ras GTPase-Activating Protein-Binding Protein 1; GAP SH3 Domain-Binding Protein 1; ATP-Dependent DNA Helicase VIII; DNA Helicase VIII; G3BP-1; RasGAP-Associated Endoribonuclease G3BP; GAP Binding Protein; EC 3.6.4.12; EC 3.6.4.13; HDH VIII; EC 3.6.1
About Gene Symbol
G3BP1 (G3BP Stress Granule Assembly Factor 1) is a GTPase-activating protein involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cel
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