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KD-Validated Anti-NF-kB p65 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1846
Application
WB, FC, ICC
Uniprot Id
Q04206
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-NF-kB p65 antibody (Cat#ABG1846). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NF-kB p65 antibody (Cat#ABG1846, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-NF-kB p65 antibody (Cat#ABG1846). NF-kB p65 expression in wild type (WT) and NF-kB p65 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NF-kB p65 antibody (Cat#ABG1846, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of NF-kB p65 expression in HepG2 cells using NF-kB p65 antibody (Cat#ABG1846, 1:2,000). Green, isotype control; red, NF-kB p65.
  • Immunocytochemical staining of HepG2 cells with NF-kB p65 antibody (Cat#ABG1846, 1:1,000). Nuclei were stained blue with DAPI; NF-kB p65 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1846-100 3 days 100 µL $499.00
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ABG1846-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-NF-kB p65 Rabbit Monoclonal Antibody
Accession (Primary)Q04206
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1490
Immunogen
A synthesized peptide derived from human NF-κB p65
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 60 kDa , observed, 65 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:2,000-1:10,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q04206 )

Gene NameRELA
Other Gene Names
RELA; RELA Proto-Oncogene, NF-KB Subunit; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells; NFKB3; P65; V-Rel Avian Reticuloendotheliosis Viral Oncogene Homolog A; Nuclear Factor NF-Kappa-B P65 Subunit; Transcription Factor P65; NF-Kappa-B Transcription Factor P65; NF-Kappa-B; P65delta3; AIF3BL3; CMCU
About Gene Symbol
RELA (RELA Proto-Oncogene, NF-KB Subunit) is a proto-oncogene involved in NF-κB signaling and angiogenesis. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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