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KD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1854
Application
WB, FC, ICC
Uniprot Id
P24864
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-cyclin E1 antibody (Cat#ABG1854). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin E1 antibody (Cat#ABG1854, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-cyclin E1 antibody (Cat#ABG1854). Cyclin E1 expression in wild-type (WT) and cyclin E1 (CCNE1) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin E1 antibody (Cat#ABG1854, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Cyclin E1 expression in HeLa cells using anti-Cyclin E1 antibody (Cat#ABG1854, 1:2,000). Green, isotype control; red, Cyclin E1.
  • Immunocytochemical staining of Hela cells with cyclin E1 antibody (Cat#ABG1854, 1:1,000). Nuclei were stained blue with DAPI; Cyclin E1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1854-100 3 days 100 µL $499.00
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ABG1854-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin E1 Rabbit Monoclonal Antibody
Accession (Primary)P24864
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB4745
Immunogen
A synthesized peptide derived from human Cyclin E1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 47 kDa, observed, 47 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P24864 )

Gene NameCCNE1
Other Gene Names
CCNE1; Cyclin E1; CCNE; G1/S-Specific Cyclin-E1; Cyclin Es; Cyclin Et; PCCNE1
About Gene Symbol
CCNE1 (Cyclin E1) is a protein kinase and signaling regulator involved in cell cycle regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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