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KD-Validated Anti-Cyclin E2 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1855
Application
WB, ICC
Uniprot Id
O96020
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-cyclin E2 antibody (Cat#ABG1855). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cyclin E2 antibody (Cat#ABG1855, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-cyclin E2 antibody (Cat#ABG1855). Cyclin E2 expression in wild-type (WT) and cyclin E2 (CCNE2) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cyclin E2 antibody (Cat#ABG1855, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of Hela cells with cyclin E2 antibody (Cat#ABG1855, 1:1,000). Nuclei were stained blue with DAPI; Cyclin E2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1855-100 3 days 100 µL $499.00
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ABG1855-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cyclin E2 Rabbit Monoclonal Antibody
Accession (Primary)O96020
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB4750
Immunogen
A synthesized peptide derived from human Cyclin E2
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 47 kDa, observed, 47 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O96020 )

Gene NameCCNE2
Other Gene Names
CCNE2; Cyclin E2; CYCE2; G1/S-Specific Cyclin-E2
About Gene Symbol
CCNE2 (Cyclin E2) is a protein kinase and signaling regulator involved in cell cycle regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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