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KD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1856
Application
WB, FC, ICC
Uniprot Id
O75794
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-CDC123 antibody (Cat#ABG1856). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC123 antibody (Cat#ABG1856, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-CDC123 antibody (Cat#ABG1856). CDC123 expression in wild-type (WT) and CDC123 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-CDC123 antibody (Cat#ABG1856, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of CDC123 expression in HAP-1 cells using anti-CDC123 antibody (Cat#ABG1856, 1:2,000). Green, isotype control; red,CDC123.
  • Immunocytochemical staining of HAP1 cells with anti-CDC123 antibody (Cat#ABG1856, 1:1,000). Nuclei were stained blue with DAPI;CDC123 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1856-100 3 days 100 µL $499.00
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ABG1856-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Cell Division Cycle 123 Rabbit Monoclonal Antibody
Accession (Primary)O75794
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB4760
Immunogen
A synthesized peptide derived from human CDC123
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 39 kDa, observed, 39 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O75794 )

Gene NameCDC123
Other Gene Names
CDC123; Cell Division Cycle 123; D123; Cell Division Cycle Protein 123 Homolog; C10orf7; HT-1080; PZ32; Cell Division Cycle 123 Homolog (S. Cerevisiae); Chromosome 10 Open Reading Frame 7; Cell Division Cycle 123 Homolog; Protein D123; C10ORF7
About Gene Symbol
CDC123 (Cell Division Cycle 123) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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