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KD-Validated Anti-MCM5 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1871
Application
WB, FC, ICC
Uniprot Id
P33992
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MCM5 antibody (Cat#ABG1871). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MCM5 antibody (Cat#ABG1871, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MCM5 antibody (Cat#ABG1871). MCM5 expression in wild-type (WT) and MCM5 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-MCM5 antibody (Cat#ABG1871, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MCM5 expression in HepG2 cells using anti-MCM5 antibody (Cat#ABG1871, 1:2,000). Green, isotype control; red, MCM5.
  • Immunocytochemical staining of HepG2 cells with anti-MCM5 antibody (Cat#ABG1871, 1:1,000). Nuclei were stained blue with DAPI; MCM5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1871-100 3 days 100 µL $499.00
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ABG1871-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MCM5 Rabbit Monoclonal Antibody
Accession (Primary)P33992
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB4965
Immunogen
A synthesized peptide derived from human MCM5
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 82 kDa, observed, 82 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P33992 )

Gene NameMCM5
Other Gene Names
MCM5; Minichromosome Maintenance Complex Component 5; CDC46; DNA Replication Licensing Factor MCM5; CDC46 Homolog; P1-CDC46; MCM5 Minichromosome MaintenanceDeficient 5, Cell Division Cycle 46 (S. Cerevisiae); Minichromosome Maintenance Deficient (S. Cerevisiae) 5 (Cell Division Cycle 46); MCM5 Minichromosome Maintenance Deficient 5, Cell Division Cycle 46; Minichromosome Maintenance Deficient 5 (Cell Division Cycle 46); EC 3.6.4.12; MGORS8
About Gene Symbol
MCM5 (Minichromosome Maintenance Complex Component 5) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechani
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