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KD-Validated Anti-Pro-Apoptotic WT1 Regulator Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1874
Application
WB, FC, ICC
Uniprot Id
Q96IZ0
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-PAWR antibody (Cat#ABG1874). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAWR antibody (Cat#ABG1874, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-PAWR antibody (Cat#ABG1874). PAWR expression in wild-type (WT) and PAWR shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PAWR antibody (Cat#ABG1874, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of PAWR expression in HepG2 cells using anti-PAWR antibody (Cat#ABG1874, 1:2,000). Green, isotype control; red, PAWR.
  • Immunocytochemical staining of HepG2 cells with anti-PAWR antibody (Cat#ABG1874, 1:1,000). Nuclei were stained blue with DAPI; PAWR was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1874-100 3 days 100 µL $499.00
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ABG1874-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Pro-Apoptotic WT1 Regulator Rabbit Monoclonal Antibody
Accession (Primary)Q96IZ0
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB5010
Immunogen
A synthesized peptide derived from human PAR4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 37 kDa, observed, 41 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q96IZ0 )

Gene NamePAWR
Other Gene Names
PAWR; Pro-Apoptotic WT1 Regulator; Par-4; PAR4; PRKC Apoptosis WT1 Regulator Protein; PRKC, Apoptosis, WT1, Regulator; Prostate Apoptosis Response-4; Prostate Apoptosis Response Protein PAR-4; Prostate Apoptosis Response Protein 4; Prostate Apoptosis Response 4 Protein; Transcriptional Repressor PAR4; WT1-Interacting Protein
About Gene Symbol
PAWR (Pro-Apoptotic WT1 Regulator) is a transcription factor involved in apoptosis. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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