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KD-Validated Anti-TIMM50 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1881
Application
WB, FC, ICC
Uniprot Id
Q3ZCQ8
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-TIMM50 antibody (Cat#ABG1881). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TIMM50 antibody (Cat#ABG1881, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-TIMM50 antibody (Cat#ABG1881). TIMM50 expression in wild-type (WT) and TIMM50 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TIMM50 antibody (Cat#ABG1881, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of TIMM50 expression in HepG2 cells using anti-TIMM50 antibody (Cat#ABG1881, 1:2,000). Green, isotype control; red, TIMM50.
  • Immunocytochemical staining of HepG2 cells with anti-TIMM50 antibody (Cat#ABG1881, 1:1,000). Nuclei were stained blue with DAPI; TIMM50 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1881-100 3 days 100 µL $499.00
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ABG1881-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-TIMM50 Rabbit Monoclonal Antibody
Accession (Primary)Q3ZCQ8
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB5300
Immunogen
A synthesized peptide derived from human TIM50
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 40 kDa; Observed, 36 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q3ZCQ8 )

Gene NameTIMM50
Other Gene Names
TIMM50; Translocase Of Inner Mitochondrial Membrane 50; TIM50; TIM50L; Mitochondrial Import Inner Membrane Translocase Subunit TIM50; Translocase Of Inner Mitochondrial Membrane 50 Homolog (Yeast); Homolog Of Yeast Tim50; Tim50-Like Protein; MGCA9
About Gene Symbol
TIMM50 (Translocase Of Inner Mitochondrial Membrane 50) is a membrane receptor involved in receptor-mediated signaling. It is an important research target in cell signaling, drug discovery, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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