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KD-Validated Anti-ALPP Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1888
Application
WB, FC, ICC
Uniprot Id
P05187
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ALPP antibody (Cat#ABG1888). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ALPP antibody (Cat#ABG1888, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ALPP antibody (Cat#ABG1888). ALPP expression in wild type (WT) and ALPP shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ALPP antibody (Cat#ABG1888, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ALPP expression in HepG2 cells using anti-ALPP antibody (Cat#ABG1888, 1:2,000). Green, isotype control; red, ALPP.
  • Immunocytochemical staining of HepG2 cells with anti-ALPP antibody (Cat#ABG1888, 1:1,000). Nuclei were stained blue with DAPI; ALPP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1888-100 3 days 100 µL $499.00
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ABG1888-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ALPP Rabbit Monoclonal Antibody
Accession (Primary)P05187
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names24GB5430
Immunogen
A synthesized peptide derived from human Placental alkaline phosphatase (phospho LAP)
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 58 kDa, observed, 63 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P05187 )

Gene NameALPP
Other Gene Names
ALPP; Alkaline Phosphatase, Placental; PLAP; PALP; Alkaline Phosphatase, Placental Type; Alkaline Phosphatase Regan Isozyme; Placental Alkaline Phosphatase 1; EC 3.1.3.1; PLAP-1; Alkaline Phosphatase, Placental (Regan Isozyme); Intestinal-Type Alkaline Phosphatase; Intestinal Alkaline Phosphatase; Placental Alkaline Phosphatase; Alkaline Phosphomonoesterase; Glycerophosphatase; Regan Isozyme; ALPI; ALP; IAP
About Gene Symbol
ALPP (Alkaline Phosphatase, Placental) is a protein phosphatase involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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