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KD-Validated Anti-Glutaminase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG1918
Application
WB, FC, ICC
Uniprot Id
O94925
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Glutaminase antibody (Cat#ABG1918). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutaminase antibody (Cat#ABG1918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Glutaminase antibody (Cat#ABG1918). Glutaminase expression in wild type (WT) and glutaminase shRNA knockdown (KD) Hela cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Glutaminase antibody (Cat#ABG1918, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of Glutaminase expression in HepG2 cells using Glutaminase antibody (Cat#ABG1918, 1:2,000). Green, isotype control; red, Glutaminase.
  • Immunocytochemical staining of HepG2 cells with Glutaminase antibody (Cat#ABG1918, 1:1,000). Nuclei were stained blue with DAPI; Glutaminase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1918-100 3 days 100 µL $499.00
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ABG1918-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Glutaminase Rabbit Monoclonal Antibody
Accession (Primary)O94925
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB 1720
Immunogen
A synthesized peptide derived from human Glutaminase
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 73 kDa , observed, 55-65 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( O94925 )

Gene NameGLS
Other Gene Names
GLS; Glutaminase; GLS1; K-Glutaminase; KIAA0838; GAC; GAM; KGA; Glutaminase Kidney Isoform, Mitochondrial; L-Glutamine Amidohydrolase; Glutaminase C; EC 3.5.1.2; Glutaminase, Phosphate; Activated; CASGID; EIEE71; AAD20; DEE71; GDPAG
About Gene Symbol
GLS (Glutaminase) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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