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KD-Validated Anti-NEFM Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1923
Application
WB, FC
Uniprot Id
P07197
Reactivity
Human
Isotype
Mouse IgG1
 
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  • Western blotting analysis using anti-neurofilament medium chain antibody (Cat#ABG1923). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-neurofilament medium chain antibody (Cat#ABG1923, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-neurofilament medium chain antibody (Cat#ABG1923). Neurofilament medium chain expression in wild type (WT) and neurofilament medium chain (NEFM) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-neurofilament medium chain antibody (Cat#ABG1923, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of Neurofilament medium chain expression in HeLa cells using anti-Neurofilament medium chain antibody (Cat#ABG1923, 1:2,000). Green, isotype control; red, Neurofilament medium chain.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1923-100 3 days 100 µL $499.00
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ABG1923-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-NEFM Mouse Monoclonal Antibody
Accession (Primary)P07197
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone Names24GB5825
Immunogen
Recombinant protein of human NEFM
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 102 kDa, observed, 150 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:2,500-1:5,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( P07197 )

Gene NameNEFM
Other Gene Names
NEFM; Neurofilament Medium Chain; NF-M; NFM; NEF3; Neurofilament, Medium Polypeptide 150kDa; Neurofilament Medium Polypeptide; Neurofilament Triplet M Protein; 160 KDa Neurofilament Protein; Neurofilament 3; Neurofilament, Medium Polypeptide; Neurofilament-3 (150 KD Medium); Neurofilament Medium
About Gene Symbol
NEFM (Neurofilament Medium Chain) is a structural cytoskeletal protein involved in cytoskeletal organization. It is an important research target in cell biology, cancer metastasis, and cytoskeletal research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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