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KD-Validated Anti-AKR1A1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1927
Application
WB, FC, ICC
Uniprot Id
P14550
Reactivity
Human, Mouse
Isotype
Mouse IgG2b
 
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  • Western blotting analysis using anti-AKR1A1 antibody (Cat#ABG1927). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKR1A1 antibody (Cat#ABG1927, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-AKR1A1 antibody (Cat#ABG1927). AKR1A1 expression in wild type (WT) and AKR1A1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AKR1A1 antibody (Cat#ABG1927, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of AKR1A1 expression in HepG2 cells using anti-AKR1A1 antibody (Cat#ABG1927, 1:2,000). Green, isotype control; red, AKR1A1.
  • Immunocytochemical staining of HepG2 cells with anti-AKR1A1 antibody (Cat#ABG1927, 1:1,000). Nuclei were stained blue with DAPI; AKR1A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1927-100 3 days 100 µL $499.00
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ABG1927-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-AKR1A1 Mouse Monoclonal Antibody
Accession (Primary)P14550
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG2b
Clone Names24GB6220
Immunogen
Recombinant protein of human AKR1A1
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 37 kDa, observed, 37 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P14550 )

Gene NameAKR1A1
Other Gene Names
AKR1A1; Aldo-Keto Reductase Family 1 Member A1; ALR; Aldehyde Reductase; DD3; Dihydrodiol Dehydrogenase 3; Glucuronolactone Reductase; Glucuronate Reductase; EC 1.1.1.2; ALDR1; Aldo-Keto Reductase Family 1, Member A1 (Aldehyde Reductase); Epididymis Secretory Sperm Binding Protein Li 165mP; Epididymis Secretory Protein Li 6; Alcohol Dehydrogenase [NADP(+)]; Alcohol Dehydrogenase; EC 1.1.1.372; HEL-S-165mP; EC 1.1.1.54; EC 1.1.1.19; EC 1.1.1.20; EC 1.1.1; HEL-S-6; ARM
About Gene Symbol
AKR1A1 (Aldo-Keto Reductase Family 1 Member A1) is a metabolic dehydrogenase involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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