KD-Validated Anti-ARHGDIA Mouse Monoclonal Antibody

Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#ABG1930). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#ABG1930, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Western blotting analysis using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#ABG1930). Rho GDP dissociation inhibitor alpha expression in wild type (WT) and Rho GDP dissociation inhibitor alpha (ARHGDIA) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#ABG1930, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Flow cytometric analysis of Rho GDP dissociation inhibitor alpha expression in HepG2 cells using anti-Rho GDP dissociation inhibitor alpha antibody (Cat#ABG1930, 1:2,000). Green, isotype control; red, Rho GDP dissociation inhibitor alpha.

Immunocytochemical staining of HepG2 cells with anti-Rho GDP dissociation inhibitor alpha antibody (Cat#ABG1930, 1:1,000). Nuclei were stained blue with DAPI; Rho GDP dissociation inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 µm.