KD-Validated Anti-CDCA8 Mouse Monoclonal Antibody

Western blotting analysis using anti-cell division cycle associated 8 antibody (Cat#ABG1951). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cell division cycle associated 8 antibody (Cat#ABG1951, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Western blotting analysis using anti-cell division cycle associated 8 antibody (Cat#ABG1951). cell division cycle associated 8 expression in wild type (WT) and cell division cycle associated 8 (CDCA8) knockdown (KD) HSHC cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cell division cycle associated 8 antibody (Cat#ABG1951, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.

Flow cytometric analysis of Cell division cycle associated 8 expression in HAP-1 cells using anti-Cell division cycle associated 8 antibody (Cat#ABG1951, 1:2,000). Green, isotype control; red, Cell division cycle associated 8.

Immunocytochemical staining of HAP-1 cells with Cell division cycle associated 8 antibody (Cat#ABG1951, 1:1,000). Nuclei were stained blue with DAPI; Cell division cycle associated 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.