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KD-Validated Anti-Creatine Kinase B Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1987
Application
WB, FC, ICC
Uniprot Id
P12277
Reactivity
Human
Isotype
Mouse IgG1
 
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  • Western blotting analysis using anti-creatine kinase B antibody (Cat#ABG1987). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-creatine kinase B antibody (Cat#ABG1987, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-creatine kinase B antibody (Cat#ABG1987). Creatine kinase B expression in wild-type (WT) and creatine kinase B (CKB) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-creatine kinase B antibody (Cat#ABG1987, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of Creatine kinase B expression in HAP-1 cells using anti-Creatine kinase B antibody (Cat#ABG1987, 1:2,000). Green, isotype control; red, Creatine kinase B.
  • Immunocytochemical staining of HAP1 cells with anti-Creatine kinase B antibody (Cat#ABG1987, 1:1,000). Nuclei were stained blue with DAPI;Creatine kinase B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1987-100 3 days 100 µL $499.00
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ABG1987-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Creatine Kinase B Mouse Monoclonal Antibody
Accession (Primary)P12277
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone Names24GB7005
Immunogen
Recombinant protein of human Creatine Kinase B
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 43 kDa, observed, 42 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P12277 )

Gene NameCKB
Other Gene Names
CKB; Creatine Kinase B; CKBB; Creatine Phosphokinase B-Type; Creatine Kinase Brain-Type; Creatine Kinase B Chain; Creatine Kinase B-Type; Brain Creatine Kinase; EC 2.7.3.2; CPK-B; B-CK; Epididymis Secretory Protein Li 29; Epididymis Luminal Protein 211; Creatine Kinase, Brain; Creatine Kinase Brain; HEL-S-29; EC 2.7.3; HEL-211; BCK
About Gene Symbol
CKB (Creatine Kinase B) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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