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KD-Validated Anti-Sp1 Transcription Factor Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1991
Application
WB, FC, ICC
Uniprot Id
P08047
Reactivity
Human
 
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  • Western blotting analysis using anti-SP1 antibody (Cat#ABG1991). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SP1 antibody (Cat#ABG1991, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-SP1 antibody (Cat#ABG1991). SP1 expression in wild-type (WT) and SP1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SP1 antibody (Cat#ABG1991, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of SP1 expression in HAP-1 cells using anti-SP1 antibody (Cat#ABG1991, 1:2,000). Green, isotype control; red, SP1.
  • Immunocytochemical staining of HAP1 cells with anti-SP1 antibody (Cat#ABG1991, 1:1,000). Nuclei were stained blue with DAPI; SP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1991-100 3 days 100 µL $499.00
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ABG1991-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Sp1 Transcription Factor Mouse Monoclonal Antibody
Accession (Primary)P08047
Host SpeciesMouse
ClonalityMonoclonal
Clone Names24GB7095
Immunogen
Recombinant protein of human SP1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 81 kDa, observed, 90 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P08047 )

Gene NameSP1
Other Gene Names
SP1; Sp1 Transcription Factor; Transcription Factor Sp1; Specificity Protein 1; TSFP1
About Gene Symbol
SP1 (Sp1 Transcription Factor) is a transcription factor involved in gene transcription and regulation. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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