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KD-Validated Anti-Ring-Box 1 Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1996
Application
WB, FC, ICC
Uniprot Id
P62877
Reactivity
Human, Mouse, Rat
 
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  • Western blotting analysis using anti-ring-box 1 antibody (Cat#ABG1996). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ring-box 1 antibody (Cat#ABG1996, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-ring-box 1 antibody (Cat#ABG1996). Ring-box 1 expression in wild-type (WT) and ring-box 1 (RBX1) shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ring-box 1 antibody (Cat#ABG1996, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of Ring-box 1 expression in C2C12 cells using anti-Ring-box 1 antibody (Cat#ABG1996, 1:2,000). Green, isotype control; red, Ring-box 1.
  • Immunocytochemical staining of C2C12 cells with anti-Ring-box 1 antibody (Cat#ABG1996, 1:1,000). Nuclei were stained blue with DAPI;Ring-box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1996-100 3 days 100 µL $499.00
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ABG1996-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Ring-Box 1 Mouse Monoclonal Antibody
Accession (Primary)P62877
Host SpeciesMouse
ClonalityMonoclonal
Clone Names24GB7170
Immunogen
Recombinant protein of human Rbx1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 12 kDa, observed, 17 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P62877 )

Gene NameRBX1
Other Gene Names
RBX1; Ring-Box 1; RNF75; ROC1; Regulator Of Cullins 1; BA554C12.1; E3 Ubiquitin-Protein Transferase RBX1; E3 Ubiquitin-Protein Ligase RBX1; RING Finger Protein 75; RING-Box Protein 1; Ring-Box 1, E3 Ubiquitin Protein Ligase; ZYP Protein; Protein ZYP; EC 2.3.2.27; EC 2.3.2.32; Rbx1
About Gene Symbol
RBX1 (Ring-Box 1) is a ubiquitin E3 ligase involved in ubiquitin-proteasome pathway. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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