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KD-Validated Anti-EZH2 Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG1998
Application
WB, FC, ICC
Uniprot Id
Q15910
Reactivity
Human
 
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  • Western blotting analysis using anti-EZH2 antibody (Cat#ABG1998). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EZH2 antibody (Cat#ABG1998, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-EZH2 antibody (Cat#ABG1998). EZH2 expression in wild-type (WT) and EZH2 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-EZH2 antibody (Cat#ABG1998, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of EZH2 expression in HAP-1 cells using anti-EZH2 antibody (Cat#ABG1998, 1:2,000). Green, isotype control; red, EZH2.
  • Immunocytochemical staining of HAP-1 cells with anti-EZH2 antibody (Cat#ABG1998, 1:1,000). Nuclei were stained blue with DAPI; EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG1998-100 3 days 100 µL $499.00
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ABG1998-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-EZH2 Mouse Monoclonal Antibody
Accession (Primary)Q15910
Host SpeciesMouse
ClonalityMonoclonal
Clone Names24GB7200
Immunogen
Recombinant protein of human EZH2
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 85 kDa, observed, 98 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q15910 )

Gene NameEZH2
Other Gene Names
EZH2; Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit 2; ENX-1; KMT6; KMT6A; Histone-Lysine N-Methyltransferase EZH2; Lysine N-Methyltransferase 6; Enhancer Of Zeste Homolog 2; EZH1; Enhancer Of Zeste (Drosophila) Homolog 2; Enhancer Of Zeste Homolog 2 (Drosophila); EC 2.1.1.356; EC 2.1.1.43; EC 2.1.1; EZH2b; ENX1; WVS2; WVS
About Gene Symbol
EZH2 (Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit 2) is a methyltransferase involved in chromatin remodeling and epigenetics. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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