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KD-Validated Anti-DNM1L Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG2034
Application
WB, FC, ICC
Uniprot Id
O00429
Reactivity
Human, Mouse, Rat
Isotype
Mouse IgG2b
 
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  • Western blotting analysis using anti-dynamin 1 like antibody (Cat#ABG2034). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-dynamin 1 like antibody (Cat#ABG2034, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-dynamin 1 like antibody (Cat#ABG2034). Dynamin 1 like expression in wild type (WT) and dynamin 1 like (DNM1L) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-dynamin 1 like antibody (Cat#ABG2034, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of Dynamin 1 like expression in HepG2 cells using anti-Dynamin 1 like antibody (Cat#ABG2034, 1:1,000). Green, isotype control; red, Dynamin 1 like.
  • Immunocytochemical staining of HepG2 cells with anti-Dynamin 1 like protein antibody (Cat#ABG2034, 1:500). Nuclei were stained blue with DAPI; Dynamin 1 like was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2034-100 3 days 100 µL $499.00
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ABG2034-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-DNM1L Mouse Monoclonal Antibody
Accession (Primary)O00429
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG2b
Clone Names24GB8215
Immunogen
Recombinant protein of human DNM1L
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 82 kDa, observed, 74 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:1,000Immunocytochemistry (IC): 1:100-1:500

Target Information: ( O00429 )

Gene NameDNM1L
Other Gene Names
DNM1L; Dynamin 1 Like; DRP1; DVLP; HDYNIV; DYMPLE; Dynamin Family Member Proline-Rich Carboxyl-Terminal Domain Less; Dynamin-Related Protein 1; Dnm1p/Vps1p-Like Protein; Dynamin-Like Protein IV; Dynamin-1-Like Protein; Dynamin-Like Protein 4; EC 3.6.5.5; VPS1; DLP1; Dynamin-Like Protein; Dymple; HdynIV; EMPF1; EMPF; OPA5
About Gene Symbol
DNM1L (Dynamin 1 Like) is a dynamin GTPase involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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