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KD-Validated Anti-PCAF Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2049
Application
WB, FC, ICC
Uniprot Id
Q92831
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-PCAF antibody (Cat#ABG2049). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PCAF antibody (Cat#ABG2049, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-PCAF antibody (Cat#ABG2049).PCAF expression in wild type (WT) and PCAF shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PCAF antibody (Cat#ABG2049, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of PCAF expression in HT-1080 cells using PCAF antibody (Cat#ABG2049, 1:2,000). Green, isotype control; red, PCAF.
  • Immunocytochemical staining of HT-1080 cells with PCAF antibody (Cat#ABG2049, 1:1,000). Nuclei were stained blue with DAPI; PCAF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2049-100 3 days 100 µL $499.00
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ABG2049-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-PCAF Rabbit Monoclonal Antibody
Accession (Primary)Q92831
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB910
Immunogen
A synthesized peptide derived from human PCAF
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 93 kDa, observed, 93 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q92831 )

Gene NameKAT2B
Other Gene Names
Lysine Acetyltransferase 2B; P/CAF; P300/CBP-Associated Factor; PCAF; Spermidine Acetyltransferase KAT2B; Histone Acetyltransferase KAT2B; K(Lysine) Acetyltransferase 2B; Histone Acetyltransferase PCAF; Histone Acetylase PCAF; EC 2.3.1.48; GCN5L; GCN5; CREBBP-Associated Factor; EC 2.3.1.57; CAF
About Gene Symbol
KAT2B (Lysine Acetyltransferase 2B) is a histone acetyltransferase involved in chromatin remodeling and epigenetics. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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