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KD-Validated Anti-Integrin β 1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2054
Application
WB, FC, ICC
Uniprot Id
P05556
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Integrin β 1 antibody (Cat#ABG2054). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Integrin β 1 antibody (Cat#ABG2054, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Integrin beta 1 antibody (Cat#ABG2054). Integrin beta 1 expression in wild type (WT) and Integrin beta 1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Integrin beta 1 antibody (Cat#ABG2054,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Flow cytometric analysis of Integrin beta 1 expression in HT-1080 cells using Integrin beta 1 antibody (Cat#ABG2054, 1:2,000). Green, isotype control; red, Integrin beta 1.
  • Immunocytochemical staining of HT-1080 cells with Integrin beta 1 antibody (Cat#ABG2054, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2054-100 3 days 100 µL $499.00
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ABG2054-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Integrin β 1 Rabbit Monoclonal Antibody
Accession (Primary)P05556
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB895
Immunogen
A synthesized peptide derived from human Integrin beta 1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 88 kDa , observed, 130-150 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P05556 )

Gene NameITGB1
Other Gene Names
Integrin Subunit Beta 1; GPIIA; MSK12; CD29; FNRB; MDF2; Integrin, Beta 1 (Fibronectin Receptor, Beta Polypeptide, Antigen CD29 Includes MDF2, MSK12); Glycoprotein IIa; Integrin Beta-1; Very Late Activation Protein, Beta Polypeptide; Fibronectin Receptor Subunit Beta; Integrin VLA-4 Beta Subunit; VLA-4 Subunit Beta; Integrin Beta 1; CD29 Antigen; VLA-BETA; VLAB
About Gene Symbol
ITGB1 (Integrin Subunit Beta 1) is an integrin cell adhesion receptor involved in adaptive immunity. It is an important research target in cell signaling, drug discovery, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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