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KD-Validated Anti-FumaRate HydRatase Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG2055
Application
WB, FC, ICC
Uniprot Id
P07954
Reactivity
Human, Mouse, Rat
Isotype
Mouse IgG1
 
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  • Western blotting analysis using anti-fumarate hydratase antibody (Cat#ABG2055). Total cell lysates (5 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fumarate hydratase antibody (Cat#ABG2055, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-fumarate hydratase antibody (Cat #ABG2055). Fumarate hydratase expression in wild-type (WT) and fumarate hydratase (FH) shRNA knockdown (KD) HeLa cells with 5 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-fumarate hydratase antibody (Cat #ABG2055, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively.
  • Flow cytometric analysis of Fumarate hydratase expression in HepG2 cells using anti-Fumarate hydratase antibody (Cat#ABG2055, 1:2,000). Green, isotype control; red, Fumarate hydratase.
  • Immunocytochemical staining of HepG2 cells with anti-Fumarate hydratase associated protein antibody (Cat#ABG2055, 1:1,000). Nuclei were stained blue with DAPI; Fumarate hydratase associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2055-100 3 days 100 µL $499.00
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ABG2055-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-FumaRate HydRatase Mouse Monoclonal Antibody
Accession (Primary)P07954
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone Names24GB13010
Immunogen
Recombinant protein of human FH
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 55 kDa, observed, 45 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P07954 )

Gene NameFH
Other Gene Names
FH; FumaRate HydRatase; Fumarase; FumaRate HydRatase, Mitochondrial; EC 4.2.1.2 HsFH; Epididymis Secretory Sperm Binding Protein; HLRCC; MCUL1; FMRD; LRCC; MCL
About Gene Symbol
FH (FumaRate HydRatase) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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