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KD-Validated Anti-Sirtuin 5 Mouse Monoclonal Antibody

Mouse monoclonal antibody

 
Catalog #
ABG2080
Application
WB, FC, ICC
Uniprot Id
Q9NXA8
Reactivity
Human
Isotype
Mouse IgG1
 
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  • Western blotting analysis using anti-sirtuin 5 antibody (Cat#ABG2080). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-sirtuin 5 antibody (Cat#ABG2080, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Western blotting analysis using anti-sirtuin 5 antibody (Cat#ABG2080). Sirtuin 5 expression in wild-type (WT) and sirtuin 5 (SIRT5) shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-sirtuin 5 antibody (Cat#ABG2080, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody respectively.
  • Flow cytometric analysis of Sirtuin 5 expression in HepG2 cells using anti-Sirtuin 5 antibody (Cat#ABG2080, 1:2,000). Green, isotype control; red, Sirtuin 5.
  • Immunocytochemical staining of HepG2 cells with anti-Sirtuin 5 associated protein antibody (Cat#ABG2080, 1:1,000). Nuclei were stained blue with DAPI; Sirtuin 5 associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2080-100 3 days 100 µL $499.00
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ABG2080-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Sirtuin 5 Mouse Monoclonal Antibody
Accession (Primary)Q9NXA8
Host SpeciesMouse
ClonalityMonoclonal
IsotypeMouse IgG1
Clone Names24GB13235
Immunogen
Recombinant protein of human SIRT5
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 34 kDa, observed, 30 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:500-1:2,500Flow Cytometry (FCM): 1:2,000Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q9NXA8 )

Gene NameSIRT5
Other Gene Names
SIRT5; Sirtuin 5; NAD-Dependent Protein Deacylase Sirtuin-5, Mitochondrial; SIR2L5; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 5 (S. Cerevisiae); Sirtuin (Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog) 5; NAD-Dependent Lysine Demalonylase And Desuccinylase Sirtuin-5, Mitochondrial; Silent Mating Type Information Regulation 2, S.Cerevisiae, Homolog 5; NAD-Dependent Deacetylase Sirtuin-5; Regulatory Protein SIR2 Homolog 5; SIR2-Like Protein 5; Sirtuin Type 5; Sir2-Like 5; EC 2.3.1.-
About Gene Symbol
SIRT5 (Sirtuin 5) is a metabolic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets
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