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KD-Validated Anti-PRAS40 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2190
Application
WB, FC, ICC
Uniprot Id
Q96B36
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-PRAS40 antibody (Cat#ABG2190). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-pPRAS40 antibody (Cat#ABG2190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-PRAS40 antibody (Cat#ABG2190). PRAS40 expression in wild type (WT) and PRAS40 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PRAS40 antibody (Cat#ABG2190, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of PRAS40 expression in HT-1080 cells using PRAS40 antibody (Cat#ABG2190, 1:2,000). Green, isotype control; red, PRAS40.
  • Immunocytochemical staining of HT-1080 cells with PRAS40 antibody (Cat#ABG2190, 1:1,000). Nuclei were stained blue with DAPI; PRAS40 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2190-100 3 days 100 µL $499.00
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ABG2190-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-PRAS40 Rabbit Monoclonal Antibody
Accession (Primary)Q96B36
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB645
Immunogen
A synthesized peptide derived from human PRAS40
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 27 kDa , observed, 40 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q96B36 )

Gene NameAKT1S1
Other Gene Names
AKT1 Substrate 1; PRAS40; Lobe; 40 KDa Proline-Rich AKT Substrate; Proline-Rich AKT1 Substrate 1; MGC2865; Proline-Rich Akt Substrate, 40 KDa; AKT1 Substrate 1 (Proline-Rich); AKT1 Substrate 1 (Proline Rich)
About Gene Symbol
AKT1S1 (AKT1 Substrate 1) is a protein kinase and signaling regulator involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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