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KD-Validated Anti-UFM1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2194
Application
WB, FC, ICC
Uniprot Id
P61960
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-UFM1 antibody (Cat#ABG2194). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UFM1 antibody (Cat#ABG2194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-UFM1 antibody (Cat#ABG2194). UFM1 expression in wild-type (WT) and UFM1 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UFM1 antibody (Cat#ABG2194, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of UFM1 expression in C2C12 cells using anti-UFM1 antibody (Cat#ABG2194, 1:2,000). Green, isotype control; red, UFM1.
  • Immunocytochemical staining of C2C12 cells with anti-UFM1 antibody (Cat#ABG2194, 1:1,000). Nuclei were stained blue with DAPI; UFM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2194-100 3 days 100 µL $499.00
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ABG2194-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-UFM1 Rabbit Monoclonal Antibody
Accession (Primary)P61960
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB85
Immunogen
A synthesized peptide derived from human UFM1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 9 kDa; observed, 10 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P61960 )

Gene NameUFM1
Other Gene Names
UFM1; Ubiquitin Fold Modifier 1; C13orf20; Ubiquitin-Fold Modifier 1; BA131P10.1; Chromosome 13 Open Reading Frame 20; BM-002; HLD14
About Gene Symbol
UFM1 (Ubiquitin Fold Modifier 1) is a metabolic enzyme involved in ubiquitin-proteasome pathway. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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