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KD-Validated Anti-MARS1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2205
Application
WB, FC, ICC
Uniprot Id
P56192
Reactivity
Human, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MARS1 antibody (Cat#ABG2205). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MARS1 antibody (Cat#ABG2205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MARS1 antibody (Cat#ABG2205). MARS1 expression in wild-type (WT) and MARS1 shRNA knockdown (KD) HeLa cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MARS1 antibody (Cat#ABG2205, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MARS1 expression in HT-1080 cells using anti-MARS1 antibody (Cat#ABG2205, 1:2,000). Green, isotype control; red, MARS1.
  • Immunocytochemical staining of HT-1080 cells with anti-MARS1 antibody (Cat#ABG2205, 1:1,000). Nuclei were stained blue with DAPI; MARS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2205-100 3 days 100 µL $499.00
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ABG2205-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MARS1 Rabbit Monoclonal Antibody
Accession (Primary)P56192
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB185
Immunogen
A synthesized peptide derived from human MARS
Species Reactivity
Reacts WithHuman, Rat
Calculated MW
Predicted, 101 kDa; observed, 95 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( P56192 )

Gene NameMARS1
Other Gene Names
MARS1; Methionyl-TRNA Synthetase 1; MetRS; Methionine--TRNA Ligase, Cytoplasmic; SPG70; CMT2U; MARS; Methionyl-TRNA Synthetase; EC 6.1.1.10; Methionine TRNA Ligase 1, Cytoplasmic; Cytosolic Methionyl-TRNA Synthetase; ILFS2; MTRNS; ILLD; TTD9; MRS
About Gene Symbol
MARS1 (Methionyl-TRNA Synthetase 1) is a biosynthetic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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