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KD-Validated Anti-MIB1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2219
Application
WB, FC
Uniprot Id
Q86YT6
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-MIB1 antibody (Cat#ABG2219). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-MIB1 antibody (Cat#ABG2219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-MIB1 antibody (Cat#ABG2219). MIB1 expression in wild-type (WT) and MIB1 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-MIB1 antibody (Cat#ABG2219, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of MIB1 expression in HepG2 cells using anti-MIB1 antibody (Cat#ABG2219, 1:2,000). Green, isotype control; red, MIB1.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2219-100 3 days 100 µL $499.00
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ABG2219-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-MIB1 Rabbit Monoclonal Antibody
Accession (Primary)Q86YT6
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB420
Immunogen
A synthesized peptide derived from human MIB1
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 110 kDa; observed, 100 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000Flow Cytometry (FCM): 1:2,000

Target Information: ( Q86YT6 )

Gene NameMIB1
Other Gene Names
MIB1; MIB E3 Ubiquitin Protein Ligase 1; ZZANK2; DIP-1; DAPK-Interacting Protein 1; KIAA1323; ZZZ6; MIB; Zinc Finger ZZ Type With Ankyrin Repeat Domain Protein; RING-Type E3 Ubiquitin Transferase MIB1; E3 Ubiquitin-Protein Ligase MIB1; DIP1; Mindbomb E3 Ubiquitin Protein Ligase 1; Mindbomb Homolog 1 (Drosophila); Ubiquitin Ligase Mind Bomb; Mind Bomb Homolog 1; EC 2.3.2.27; EC 6.3.2; LVNC7
About Gene Symbol
MIB1 (MIB E3 Ubiquitin Protein Ligase 1) is a ubiquitin E3 ligase involved in ubiquitin-proteasome pathway. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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