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KD-Validated Anti-Phospho-KIF22 (S427) Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2240
Application
WB, ICC
Uniprot Id
Q14807
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-phospho-KIF22 (S427) antibody (Cat#ABG2240). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-KIF22 (S427) antibody (Cat#ABG2240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-phospho-KIF22 (S427) antibody (Cat#ABG2240). Phospho-KIF22 (S427) expression in wild-type (WT) and KIF22 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-KIF22 (S427) antibody (Cat#ABG2240, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Immunocytochemical staining of HepG2 cells with anti-Phospho-KIF22 (S427) antibody (Cat#ABG2240, 1:1,000). Nuclei were stained blue with DAPI; Phospho-KIF22 (S427) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2240-100 3 days 100 µL $499.00
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ABG2240-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Phospho-KIF22 (S427) Rabbit Monoclonal Antibody
Accession (Primary)Q14807
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names25GB1650
Immunogen
A synthesized peptide derived from human KIF22 around the phosphorylation site of S427
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 73 kDa; observed, 73 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( Q14807 )

Gene NameKIF22
Other Gene Names
KIF22; Kinesin Family Member 22; OBP-1; OBP-2; KNSL4; Kid; Kinesin-Like Protein KIF22; Kinesin-Like Protein 4; Origin Of Plasmid DNA Replication-Binding Protein; Kinesin-Like DNA-Binding Protein Pseudogene; Kinesin-Like DNA-Binding Protein; OriP Binding Protein; Kinesin-Like 4; A-328A3.2; SEMDJL2; OBP; KID
About Gene Symbol
KIF22 (Kinesin Family Member 22) is a kinesin motor protein involved in gene transcription and regulation. It is an important research target in gene regulation, development, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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