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KD-Validated Anti-Integrin β1/CD29 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2300
Application
WB, FC, ICC
Uniprot Id
P05556
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Integrin β1/CD29 antibody (Cat#ABG2300). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Integrin β1/CD29 antibody (Cat#ABG2300, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Integrin beta-1/CD29 antibody (Cat#ABG2300). Integrin beta-1/CD29 expression in wild type (WT) and Integrin beta-1/CD29 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Integrin beta-1/CD29 antibody (Cat#ABG2300,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Flow cytometric analysis of Integrin beta-1/CD29 expression in HT-1080 cells using Integrin beta-1/CD29 antibody (Cat#ABG2300,1:2,000). Green, isotype control; red, Integrin beta-1/CD29.
  • Immunocytochemical staining of HT-1080 cells with Integrin beta-1/CD29 antibody (Cat#ABG2300, 1:1,000). Nuclei were stained blue with DAPI; Integrin beta-1/CD29 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2300-100 3 days 100 µL $499.00
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ABG2300-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Integrin β1/CD29 Rabbit Monoclonal Antibody
Accession (Primary)P05556
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB885
Immunogen
A synthesized peptide derived from human integrin beta-1/CD29
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 88 kDa , observed, 115/135 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemical (IC): 1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( P05556 )

Gene NameITGB1
Other Gene Names
GPIIA; MSK12; CD29; FNRB; MDF2; Integrin, Beta 1 (Fibronectin Receptor, Beta Polypeptide, Antigen CD29 Includes MDF2, MSK12); Glycoprotein IIa; Integrin Beta-1; Very Late Activation Protein, Beta Polypeptide; Fibronectin Receptor Subunit Beta; Integrin VLA-4 Beta Subunit; VLA-4 Subunit Beta; Integrin Beta 1; CD29 Antigen; VLA-BETA; VLAB
About Gene Symbol
ITGB1 (Integrin Subunit Beta 1) is an integrin cell adhesion receptor involved in adaptive immunity. It is an important research target in cell signaling, drug discovery, and cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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