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KD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2309
Application
WB, FC, ICC
Uniprot Id
P49840/P49841
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-GSK3 alpha/beta antibody (Cat#ABG2309). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GSK3 alpha/beta antibody (Cat#ABG2309, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-GSK3 a/ antibody (Cat#ABG2309). GSK3 a/ expression in wildtype (WT) and GSK3 a/3 shRNA knockdown (KD) HeLa cells with 30 ug of total cell lysates. B-Tubulin serves as a loading control. The blot was incubated with anti-GSK3 a/ antibody(Cat#ABG2309, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of GSK3 alpha/beta expression in HepG2 cells using GSK3 alpha/beta antibody (Cat#ABG2309, 1:2,000). Green, isotype control; red, GSK3 alpha/beta.
  • Immunocytochemical staining of HepG2 cells with GSK3 alpha/beta antibody (Cat#ABG2309, 1:1,000). Nuclei were stained blue with DAPI; GSK3 alpha/beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2309-100 3 days 100 µL $499.00
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ABG2309-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-GSK3 alpha/beta Rabbit Monoclonal Antibody
Accession (Primary)P49840/P49841
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1100
Immunogen
Recombinant protein of human GSK3 alpha
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted , 51 kDa , observed , 51,47 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P49840/P49841 )

Gene NameGSK3A
Other Gene Names
GSK3A; Glycogen Synthase Kinase 3 Alpha; Serine/Threonine-Protein Kinase GSK3A; Glycogen Synthase Kinase-3 Alpha; EC 2.7.11.26; GSK-3 Alpha; EC 2.7.11.1; EC 2.7.11;Glycogen Synthase Kinase 3 Beta; Serine/Threonine-Protein Kinase GSK3B; Glycogen Synthase Kinase-3 Beta; EC 2.7.11.26; GSK-3 Beta; EC 2.7.11.1; EC 2.7.11
About Gene Symbol
GSK3A/GSK3B (Glycogen Synthase Kinase 3 Alpha) is a serine/threonine protein kinase involved in cell signaling and proliferation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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