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KD-Validated Anti-Smad4 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2312
Application
WB, FC, ICC
Uniprot Id
Q13485
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-Smad4 antibody (Cat#ABG2312). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smad4 antibody (Cat#ABG2312, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Western blotting analysis using anti-Smad4 antibody (Cat#ABG2312). Smad4 expression in wild type (WT) and Smad4 shRNA knockdown (KD) HT-1080 cells with 30 µg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Smad4 antibody (Cat#ABG2312,1:5,000) and HRP-conjugated goat anti rabbit secondary antibody respectively.
  • Flow cytometric analysis of Smad4 expression in C2C12 cells using Smad4 antibody (Cat#ABG2312,1:2,000). Green, isotype control; red, Smad4.
  • Immunocytochemical staining of C2C12 cells with Smad4 antibody (Cat#ABG2312, 1:1,000). Nuclei were stained blue with DAPI; Smad4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2312-100 3 days 100 µL $499.00
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ABG2312-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-Smad4 Rabbit Monoclonal Antibody
Accession (Primary)Q13485
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB975
Immunogen
A synthesized peptide derived from human Smad4
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 60 kDa , observed, 65 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemical (IC): 1:1,000 Flow Cytometry (FCM): 1:2,000

Target Information: ( Q13485 )

Gene NameSMAD4
Other Gene Names
DPC4; MADH4; Mothers Against Decapentaplegic Homolog 4; Deletion Target In Pancreatic Carcinoma 4; MAD Homolog 4; MAD, Mothers Against Decapentaplegic Homolog 4 (Drosophila); Mothers Against Decapentaplegic, Drosophila, Homolog Of, 4; SMAD, Mothers Against DPP Homolog 4 (Drosophila); Deleted In Pancreatic Carcinoma Locus 4; SMAD, Mothers Against DPP Homolog 4; Mothers Against DPP Homolog 4; SMAD 4; HSMAD4; MYHRS; Smad4; JIP
About Gene Symbol
SMAD4 (Mothers Against Decapentaplegic Homolog 4) is a transcription factor involved in TGF-β signaling. It is an important research target in cancer research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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