KD-Validated Anti-Glycogen synthase kinase 3 beta Rabbit Monoclonal Antibody

Western blotting analysis using anti-Glycogen synthase kinase 3 beta antibody (Cat#ABG2314). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glycogen synthase kinase 3 beta antibody (Cat#ABG2314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Glycogen synthase kinase 3 beta antibody (Cat#ABG2314). Glycogen synthase kinase 3 beta expression in wild type (WT) and glycogen synthase kinase 3 beta shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Glycogen synthase kinase 3 beta antibody (Cat#ABG2314, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Flow cytometric analysis of Glycogen synthase kinase 3 beta expression in HepG2 cells using Glycogen synthase kinase 3 beta antibody (Cat#ABG2314, 1:2,000). Green, isotype control; red, Glycogen synthase kinase 3 beta.

Immunocytochemical staining of HT-1080 cells with Glycogen synthase kinase 3 beta antibody (Cat#ABG2314, 1:1,000). Nuclei were stained blue with DAPI; Glycogen synthase kinase 3 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.