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KD-Validated Anti-ATP citrate lyase Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2316
Application
WB, FC, ICC
Uniprot Id
P53396
Reactivity
Human, Mouse, Rat
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-ATP citrate lyase antibody (Cat#ABG2316). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP citrate lyase antibody (Cat#ABG2316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-ATP citrate lyase antibody (Cat#ABG2316). ATP citrate lyase expression in wild type (WT) and ATP citrate lyase shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ATP citrate lyase antibody (Cat#ABG2316, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of ATP citrate lyase expression in HepG2 cells using ATP citrate lyase antibody (Cat#ABG2316, 1:2,000). Green, isotype control; red, ATP citrate lyase.
  • Immunocytochemical staining of HepG2 cells with ATP citrate lyase antibody (Cat#ABG2316, 1:1,000). Nuclei were stained blue with DAPI; ATP citrate lyase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2316-100 3 days 100 µL $499.00
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ABG2316-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-ATP citrate lyase Rabbit Monoclonal Antibody
Accession (Primary)P53396
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1620
Immunogen
A synthesized peptide derived from human ATP citrate lyase
Species Reactivity
Reacts WithHuman, Mouse, Rat
Calculated MW
Predicted, 121 kDa , observed, 121 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( P53396 )

Gene NameACLY
Other Gene Names
ACLY; ATP Citrate Lyase; ACL; ATPCL; CLATP; ATP-Citrate (Pro-S-)-Lyase; Citrate Cleavage Enzyme; ATP-Citrate Synthase; EC 2.3.3.8; ATP Citrate Synthase
About Gene Symbol
ACLY (ATP Citrate Lyase) is a biosynthetic enzyme involved in cellular metabolism. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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