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KD-Validated Anti-AGO3 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2333
Application
WB, FC, ICC
Uniprot Id
Q9H9G7
Reactivity
Human, Mouse
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-AGO3 antibody (Cat#ABG2333). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AGO3 antibody (Cat#ABG2333, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-AGO3 antibody (Cat#ABG2333). AGO3 expression in wild-type (WT) and AGO3 shRNA knockdown (KD) HT-1080 cells with 20 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-AGO3 antibody (Cat#ABG2333, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of AGO3 expression in HeLa cells using AGO3 antibody (Cat#ABG2333, 1:2,000). Green, isotype control; red, AGO3.
  • Immunocytochemical staining of Hela cells with AGO3 antibody (Cat#ABG2333, 1:1,000). Nuclei were stained blue with DAPI; AGO3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2333-100 3 days 100 µL $499.00
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ABG2333-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-AGO3 Rabbit Monoclonal Antibody
Accession (Primary)Q9H9G7
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB3930
Immunogen
A synthesized peptide derived from human EIF2C3
Species Reactivity
Reacts WithHuman, Mouse
Calculated MW
Predicted, 97 kDa , observed, 97 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:1,000

Target Information: ( Q9H9G7 )

Gene NameAGO3
Other Gene Names
AGO3; Argonaute RISC Catalytic Component 3; EIF2C3; Eukaryotic Translation Initiation Factor 2C, 3; Protein Argonaute-3; FLJ12765; HAGO3; HAgo3; Eukaryotic Translation Initiation Factor 2C; Argonaute 3, RISC Catalytic Component; EC 3.1.26.N2; Argonaute 3; Argonaute3; EIF-2C 3; EIF2C 3
About Gene Symbol
AGO3 (Argonaute RISC Catalytic Component 3) is a metabolic enzyme involved in translational regulation. It is an important research target in metabolic disease, cancer metabolism, and drug discovery and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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