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KD-Validated Anti-BubR1 Rabbit Monoclonal Antibody

Rabbit monoclonal antibody

 
Catalog #
ABG2337
Application
WB, FC, ICC
Uniprot Id
O60566
Reactivity
Human
Isotype
Rabbit IgG
 
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  • Western blotting analysis using anti-BubR1 antibody (Cat#ABG2337). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BCL11 transcription factor B antibody (Cat#ABG2337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Western blotting analysis using anti-BubR1 antibody (Cat#ABG2337). BubR1 expression in wild type (WT) and BubR1 shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-BubR1 antibody (Cat#ABG2337, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.
  • Flow cytometric analysis of BubR1 expression in HepG2 cells using BubR1 antibody (Cat#ABG2337, 1:2,000). Green, isotype control; red, BubR1.
  • Immunocytochemical staining of HepG2 cells with BubR1 antibody (Cat#ABG2337, 1:1,000). Nuclei were stained blue with DAPI; BubR1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.
 Catalog #AvailabilitySizeQuantityUnit Price Save For Later Wish List
ABG2337-100 3 days 100 µL $499.00
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ABG2337-20 3 days 20 µL $149.00
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Product Overview

NameKD-Validated Anti-BubR1 Rabbit Monoclonal Antibody
Accession (Primary)O60566
Host SpeciesRabbit
ClonalityMonoclonal
IsotypeRabbit IgG
Clone Names23GB1640
Immunogen
A synthesized peptide derived from human BubR1
Species Reactivity
Reacts WithHuman
Calculated MW
Predicted, 120 kDa ; Observed, 120 kDa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Recommended Dilution
Western blotting (WB): 1:1,000-1:5,000 Flow cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000

Target Information: ( O60566 )

Gene NameBUB1B
Other Gene Names
BUB1 Mitotic Checkpoint Serine/Threonine Kinase B; BUBR1; MAD3L; SSK1; Bub1A; Mitotic Checkpoint Serine/Threonine-Protein Kinase BUB1 Beta; MAD3/BUB1-Related Protein Kinase; Mitotic Checkpoint Kinase MAD3L; HBUBR1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog), Beta; Budding Uninhibited By Benzimidazoles 1 Homolog Beta (Yeast); Budding Uninhibited By Benzimidazoles 1 Homolog Beta; BUB1B, Mitotic Checkpoint Serine/Threonine Kinase; Protein SSK1; EC 2.7.11.1; BUB1beta; MVA1
About Gene Symbol
BUB1B (BUB1 Mitotic Checkpoint Serine/Threonine Kinase B) is a serine/threonine protein kinase involved in cell cycle regulation. It is an important research target in cancer, cell signaling, and therapeutic target research and is widely studied to elucidate cellular mechanisms and identify therapeutic targets.
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