KD-Validated Anti-Acyl-CoA dehydrogenase short chain Rabbit Monoclonal Antibody

Western blotting analysis using anti-Acyl-CoA dehydrogenase short chain antibody (Cat#ABG2342). Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acyl-CoA dehydrogenase short chain antibody (Cat#ABG2342, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Western blotting analysis using anti-Acyl-CoA dehydrogenase short chain antibody (Cat#ABG2342). Acyl-CoA dehydrogenase short chain expression in wild type (WT) and acyl-CoA dehydrogenase short chain shRNA knockdown (KD) HeLa cells with 30 µg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Acyl-CoA dehydrogenase short chain antibody (Cat#ABG2342, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody respectively.

Immunocytochemical staining of HeLa cells with Acyl-CoA dehydrogenase short chain antibody (Cat#ABG2342, 1:1,000). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 µm.